Coding

Part:BBa_K1321345:Design

Designed by: Chris N Micklem   Group: iGEM14_Imperial   (2014-10-08)


NiBP fused to dCBD with linker in RFC 25


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 174


Design Notes

The fusion protein was cloned together using the NgoMIV and AgeI sites.

Source

Our NiBP came from BBa_K1151001. dCBD with its linker were synthesized from Geneart and cloned into the psB1C3 backbone.

References